Monthly Archives: Maggio 2021

12,184 DEAD 1,196,190 Injuries: European Database of Adverse Drug Reactions for COVID-19 ‘Vaccines’…

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“Doctors of the earth, what have you done to yourselves in your practices? You do not seek to preserve life, but to destroy.” – Our Lady, March 18, 1973

MASTERS OF DECEIT
“Be known it to you that it has been written of and spoken of as 666, the masters of deceit. Satan has entered into the highest places of your government, of each nation of the world, to sow confusion, discontentment, atheism, communism, satanism, and all manner of evil.” – Jesus, October 2, 1980

THE OCTOPUS OF EVIL
“The octopus of evil having many arms now is reaching out. The evil and the agents of satan in human form have entered into the highest places in world governments and in My Son’s Church.” – Our Lady, March 18, 1977

The above Messages from Our Lady were given to Veronica Lueken at Bayside, New York. Read more

ConservativeChoiceCampaign.com reported on May 26, 2021:

by Brian Shilhavy

The European database of suspected drug reaction reports is EudraVigilance, which also tracks reports of injuries and deaths following the experimental COVID-19 “vaccines.”

Here is what EudraVigilance states about their database:

This website was launched by the European Medicines Agency in 2012 to provide public access to reports of suspected side effects (also known as suspected adverse drug reactions). These reports are submitted electronically to EudraVigilance by national medicines regulatory authorities and by pharmaceutical companies that hold marketing authorisations (licences) for the medicines.

EudraVigilance is a system designed for collecting reports of suspected side effects. These reports are used for evaluating the benefits and risks of medicines during their development and monitoring their safety following their authorisation in the European Economic Area (EEA). EudraVigilance has been in use since December 2001.

This website was launched to comply with the EudraVigilance Access Policy, which was developed to improve public health by supporting the monitoring of the safety of medicines and to increase transparency for stakeholders, including the general public.

The Management Board of the European Medicines Agency first approved the EudraVigilance Access Policy in December 2010. A revision was adopted by the Board in December 2015 based on the 2010 pharmacovigilance legislation. The policy aims to provide stakeholders such as national medicines regulatory authorities in the EEA, the European Commission, healthcare professionals, patients and consumers, as well as the pharmaceutical industry and research organisations, with access to reports on suspected side effects.

Transparency is a key guiding principle of the Agency, and is pivotal to building trust and confidence in the regulatory process. By increasing transparency, the Agency is better able to address the growing need among stakeholders, including the general public, for access to information. (Source.)

Their report through May 22, 2021 lists 12,184 deaths and 1,196,190 injuries following injections of four experimental COVID-19 shots:

From the total of injuries recorded, there are 604,744 serious injuries which equals over 50%.

“Seriousness provides information on the suspected undesirable effect; it can be classified as ‘serious’ if it corresponds to a medical occurrence that results in death, is life-threatening, requires inpatient hospitalisation, results in another medically important condition, or prolongation of existing hospitalisation, results in persistent or significant disability or incapacity, or is a congenital anomaly/birth defect.”

A Health Impact News subscriber in Europe ran the reports for each of the four COVID-19 shots we are including here. This subscriber has volunteered to do this, and it is a lot of work to tabulate each reaction with injuries and fatalities, since there is no place on the EudraVigilance system we have found that tabulates all the results.

Since we have started publishing this, others from Europe have also calculated the numbers and confirmed the totals.*

Here is the summary data through May 22, 2021.

Total reactions for the experimental mRNA vaccineTozinameran (code BNT162b2,Comirnaty) from BioNTech/ Pfizer: 5,961 deaths and 452,779 injuries to 22/05/2021

  • 13,531   Blood and lymphatic system disorders incl. 59 deaths
  • 9,828     Cardiac disorders incl. 735 deaths
  • 71           Congenital, familial and genetic disorders incl. 4 deaths
  • 5,468     Ear and labyrinth disorders incl. 3 deaths
  • 183        Endocrine disorders
  • 6,266     Eye disorders incl. 14 deaths
  • 41,214   Gastrointestinal disorders incl. 216 deaths
  • 128,031 General disorders and administration site conditions incl. 1,909 deaths
  • 327        Hepatobiliary disorders incl. 27 deaths
  • 4,802     Immune system disorders incl. 31 deaths
  • 13,948   Infections and infestations incl. 648 deaths
  • 4,821     Injury, poisoning and procedural complications incl. 81 deaths
  • 10,374   Investigations incl. 221 deaths
  • 3,354     Metabolism and nutrition disorders incl. 120 deaths
  • 65,326   Musculoskeletal and connective tissue disorders incl. 71 deaths
  • 250        Neoplasms benign, malignant and unspecified (incl cysts and polyps) incl. 15 deaths
  • 81,748   Nervous system disorders incl. 616 deaths
  • 279        Pregnancy, puerperium and perinatal conditions incl. 7 deaths
  • 88           Product issues
  • 7,978     Psychiatric disorders incl. 94 deaths
  • 1,342     Renal and urinary disorders incl. 93 deaths
  • 1,570     Reproductive system and breast disorders incl. 3 deaths
  • 18,597   Respiratory, thoracic and mediastinal disorders incl. 697 deaths
  • 21,101   Skin and subcutaneous tissue disorders incl. 53 deaths
  • 663        Social circumstances incl. 9 deaths
  • 160        Surgical and medical procedures incl. 10 deaths
  • 11,459   Vascular disorders incl. 225 deaths

Total reactions for the experimental mRNA vaccine mRNA-1273(CX-024414) from Moderna: 3,365 deaths and 72,596 injuries to 22/05/2021

  • 1,335     Blood and lymphatic system disorders incl. 22 deaths
  • 2,045     Cardiac disorders incl. 370 deaths
  • 12           Congenital, familial and genetic disorders incl. 2 deaths
  • 718        Ear and labyrinth disorders
  • 37           Endocrine disorders incl. 1 death
  • 997        Eye disorders incl. 4 deaths
  • 6,305     Gastrointestinal disorders incl. 108 deaths
  • 20,774   General disorders and administration site conditions incl. 1,480 deaths
  • 129        Hepatobiliary disorders incl. 8 deaths
  • 691        Immune system disorders incl. 4 deaths
  • 2,392     Infections and infestations incl. 183 deaths
  • 1,292     Injury, poisoning and procedural complications incl. 63 deaths
  • 1,743     Investigations incl. 77 deaths
  • 816        Metabolism and nutrition disorders incl. 64 deaths
  • 9,149     Musculoskeletal and connective tissue disorders incl. 62 deaths
  • 77           Neoplasms benign, malignant and unspecified (incl cysts and polyps) incl. 11 deaths
  • 12,314   Nervous system disorders incl. 339 deaths
  • 83           Pregnancy, puerperium and perinatal conditions
  • 11           Product issues
  • 1,375     Psychiatric disorders incl. 51 deaths
  • 468        Renal and urinary disorders incl. 40 deaths
  • 175        Reproductive system and breast disorders incl. 1 death
  • 3,513     Respiratory, thoracic and mediastinal disorders incl. 306 deaths
  • 3,726     Skin and subcutaneous tissue disorders incl. 23 deaths
  • 259        Social circumstances incl. 9 deaths
  • 235        Surgical and medical procedures incl. 26 deaths
  • 1,925     Vascular disorders

Total reactions for the experimental vaccine AZD1222/VAXZEVRIA (CHADOX1 NCOV-19) from Oxford/AstraZeneca: 2,489 deaths and 655,534 injuries to 22/05/2021

  • 7,200     Blood and lymphatic system disorders incl. 100 deaths
  • 9,748     Cardiac disorders incl. 311 deaths
  • 103        Congenital, familial and genetic disorders incl. 2 deaths
  • 6,740     Ear and labyrinth disorders
  • 217        Endocrine disorders incl. 2 deaths
  • 10,591   Eye disorders incl. 8 deaths
  • 69,826   Gastrointestinal disorders incl. 116 deaths
  • 178,037 General disorders and administration site conditions incl. 685 deaths
  • 396        Hepatobiliary disorders incl. 20 deaths
  • 2,409     Immune system disorders incl. 9 deaths
  • 13,832   Infections and infestations incl. 163 deaths
  • 5,870     Injury, poisoning and procedural complications incl. 46 deaths
  • 13,474   Investigations incl. 50 deaths
  • 8,405     Metabolism and nutrition disorders incl. 35 deaths
  • 104,075 Musculoskeletal and connective tissue disorders incl. 25 deaths
  • 222        Neoplasms benign, malignant and unspecified (incl cysts and polyps) incl. 6 deaths
  • 141,437 Nervous system disorders incl. 388 deaths
  • 156        Pregnancy, puerperium and perinatal conditions incl. 3 deaths
  • 76           Product issues
  • 12,272   Psychiatric disorders incl. 21 deaths
  • 2,264     Renal and urinary disorders incl. 20 deaths
  • 3,327     Reproductive system and breast disorders
  • 21,237   Respiratory, thoracic and mediastinal disorders incl. 278 deaths
  • 29,750   Skin and subcutaneous tissue disorders incl. 14 deaths
  • 582        Social circumstances incl. 4 deaths
  • 498        Surgical and medical procedures incl. 15 deaths
  • 12,790   Vascular disorders incl. 168 deaths

Total reactions for the experimental COVID-19 vaccine JANSSEN (AD26.COV2.S) from Johnson & Johnson: 369 deaths and 15,281 injuries to 22/05/2021

  • 145        Blood and lymphatic system disorders incl. 10 deaths
  • 264        Cardiac disorders incl. 34 deaths
  • 8             Congenital, familial and genetic disorders
  • 77           Ear and labyrinth disorders
  • 5             Endocrine disorders incl. 1 death
  • 191        Eye disorders incl. 2 deaths
  • 1,302     Gastrointestinal disorders incl. 11 deaths
  • 3,619     General disorders and administration site conditions incl. 97 deaths
  • 38           Hepatobiliary disorders incl. 2 deaths
  • 51           Immune system disorders
  • 245        Infections and infestations incl. 8 deaths
  • 209        Injury, poisoning and procedural complications incl. 6 deaths
  • 1,134     Investigations incl. 23 deaths
  • 104        Metabolism and nutrition disorders incl. 10 deaths
  • 2,368     Musculoskeletal and connective tissue disorders incl. 12 deaths
  • 12           Neoplasms benign, malignant and unspecified (incl cysts and polyps)
  • 3,051     Nervous system disorders incl. 48 death
  • 7             Pregnancy, puerperium and perinatal conditions
  • 8             Product issues
  • 181        Psychiatric disorders incl. 3 deaths
  • 69           Renal and urinary disorders incl. 4 deaths
  • 62           Reproductive system and breast disorders
  • 637        Respiratory, thoracic and mediastinal disorders incl. 29 deaths
  • 324        Skin and subcutaneous tissue disorders incl. 1 death
  • 39           Social circumstances incl. 2 deaths
  • 214        Surgical and medical procedures incl. 20 deaths
  • 917        Vascular disorders incl. 46 deaths

*These totals are estimates based on reports submitted to EudraVigilance. Totals may be much higher based on percentage of adverse reactions that are reported. Some of these reports may also be reported to the individual country’s adverse reaction databases, such as the U.S. VAERS database, and the UK Yellow Card system. The fatalities are grouped by symptoms, and some fatalities may have resulted from multiple symptoms.

https://www.tldm.org/news50/12184-dead-1196190-injuries-european-database-of-adverse-drug-reactions-for-covid-19-vaccines.htm

Bergoglio vuole abolire Summorum Pontificum

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NEWS: Summorum Pontificum, Francesco vuole abolirlo! Lo ha detto alla Plenaria CEI

Si tratta, per adesso, di notizie ancora frammentarie, da nostre plurime fonti all’interno della CEI ed episcopali, ma sembra che ieri (24.5.2021) il Papa, rivolgendosi ai vescovi italiani in apertura dei lavori dell’assemblea annuale della CEI (e in un successivo incontro con un gruppo di loro), abbia preannunciato l’imminente riforma in peggio del Motu proprio Summorum Pontificum.
 
Dopo l’ennesima messa in guardia dall’accogliere in seminario dei giovani “rigidi” (cioè fedeli alla dottrina), Francesco ha annunciato ai vescovi che è giunto alla terza stesura di un testo che reca misure restrittive in ordine alla celebrazione da parte dei sacerdoti cattolici della Messa nella forma extraordinaria liberalizzata da Benedetto XVI il quale, a suo dire, con il Summorum Pontificum voleva andare incontro solo ai lefebvriani, ma che oggi sono soprattutto i giovani preti a voler celebrare la Messa tridentina magari non sapendo neanche il latino.
 
In proposito ha raccontato di un vescovo al quale si era rivolto un giovane prete manifestandogli l’intenzione di celebrare nella forma extraordinaria. Alla domanda se sapesse il latino, il giovane prete gli ha detto che lo stava imparando. Al che il vescovo gli ha risposto che sarebbe stato meglio imparasse lo spagnolo o il vietnamita in quanto in diocesi erano molti gli ispanici e i vietnamiti.
 
A quanto si comprende, si ritornerebbe all’indulto – con previa autorizzazione del vescovo, o addirittura del Vaticano –  con tutto ciò che ne consegue e cioè una reintroduzione del divieto di celebrare secondo il Messale di San Giovanni XXIII, tantissimi dinieghi delle autorizzazioni e la pratica ghettizzazione dei sacerdoti e dei fedeli legati all’antico rito. Dopo Mose il liberatore, ritornerebbe il Faraone.
 
La  credenza che il Summorum Pontifucum sia stato redatto solo per andare incontro ai lefrebvriani è infondata e falsa: non solo perché lo si evince dal testo del Motu proprio (e dall’Universae Ecclesiae) ma a dirlo espressamente è lo stesso Benedetto XVI che a pagina 189 – 190 del libro “Ultime conversazioni” (a cura di P. Seewald, ed Corriere della Sera, si veda foto sotto) secondo cui la riabilitazione  della Messa  antica col Summorum Pontificum non deve essere assolutamente inteso come concessione alla Fraternità, ma come via perché tutta la “Chiesa preservasse la continuità interna con il suo passato. Ciò che prima era sacro non divenisse da un momento all’altro una cosa sbagliata. Adesso non c’è un’altra Messa. Sono che diverse forme dello stesso rito”.
 
Un sacerdote ci ha detto a tal proposito: “Non mi sembra strano, che i vescovi attacchino il Summorum Pontificum: dopo tutto quello della liturgia antica è il più grave, serio e attuale problema della Chiesa“.
 

Se sono arrivati già alla terza bozza vuol dire che stanno lavorando sul serio (e da tempo) per limitare e – di fatto – annullare il Summorum Pontificum. C’è quindi veramente da preoccuparsi e da pregare: Benedetto XVI avrà qualcosa da dire?
 
Vi terremo aggiornati.
 
La Redazione 

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Norvegia: il Vaccino Pfizer Responsabile della Morte di Anziani Fragili.

29 Maggio 2021 Pubblicato da Lascia il tuo commento

 

Marco Tosatti

Cari amici e nemici di Stilum Curiae, mentre la propaganda vaccinista nel nostro Paese sta raggiungendo livelli incredibili sui mass media generalisti, è interessante offrire qualche elemento di riflessione ulteriore che proviene da Paesi in cui il rispetto dei cittadini e dei loro diritti, anche a fronte di terapie sperimentali praticate su larghissima scala, è certamente più sentito. Questo articolo proviene dal British  Medicine Journal. Buona lettura. 

§§§

Il vaccino Pfizer-BioNTech covid-19 è “probabilmente” responsabile di almeno 10 morti di anziani fragili in case di cura in Norvegia, ha concluso una revisione di esperti commissionata dall’Agenzia norvegese dei medicinali.

Il gruppo di esperti è stato istituito alla fine di febbraio 2021 per esaminare la causa dei primi 100 decessi segnalati di residenti in case di cura che avevano ricevuto il vaccino Pfizer-BioNTech. All’epoca, circa 30.000 anziani residenti in case di cura erano stati vaccinati.

Anche se il tasso di mortalità nelle case di cura è generalmente molto alto e la morte di alcuni residenti nelle case di cura dopo la vaccinazione era prevista, l’Agenzia norvegese per i medicinali voleva determinare se il vaccino avesse eventualmente accelerato qualche morte e per ottenere una comprensione più chiara dei rischi e dei benefici del suo uso negli anziani fragili.

La revisione ha riferito il 19 maggio e ha concluso che un legame causale tra il vaccino Pfizer-BioNTech e la morte è stato considerato “probabile” in 10 dei 100 casi, “possibile” in 26 casi e “improbabile” in 59 casi. I restanti cinque sono stati considerati “non classificabili”. 1

Pur sottolineando una notevole incertezza intorno alle sue conclusioni, il gruppo di esperti ha riconosciuto il rischio che le reazioni avverse ai vaccini tra i pazienti molto fragili possano iniziare una cascata di complicazioni, che nel peggiore dei casi potrebbe portare a una morte anticipata.

 

“I pazienti fragili possono beneficiare della vaccinazione perché sono a grande rischio di gravi malattie e persino di morte se vengono infettati dal virus covid-19”, ha detto Sigurd Hortemo, consulente medico senior dell’Agenzia norvegese per i medicinali. “Tuttavia, il gruppo di esperti ritiene che, per alcuni di questi pazienti fragili, le reazioni avverse comuni possono aver contribuito a un decorso più grave della loro malattia”.

Il gruppo ha notato che una valutazione più approfondita dei benefici e dei rischi della vaccinazione avrebbe potuto essere fatta per alcuni anziani molto fragili, in particolare durante le prime settimane di utilizzo del vaccino.  Le persone con un’aspettativa di vita molto breve hanno poco da guadagnare dall’essere vaccinate, ha detto, notando un rischio reale che il momento della morte sarà anticipato e che sperimenteranno reazioni avverse al vaccino negli ultimi giorni della loro vita.

I benefici della vaccinazione per le persone molto fragili con un’aspettativa di vita molto breve dovrebbero quindi essere attentamente valutati rispetto ai rischi associati, e spesso potrebbe essere meglio non vaccinare, ha raccomandato il gruppo. Tuttavia, le linee guida sulla valutazione del rischio – emesse dall’Istituto norvegese di salute pubblica poco dopo le prime morti segnalate di pazienti anziani fragili dopo la vaccinazione – sono adeguate, ha detto. Queste consigliano che i medici dovrebbero valutare tali pazienti individualmente per determinare se i benefici della vaccinazione superano gli effetti collaterali. Le linee guida raccomandano che, prima della vaccinazione, i medici dovrebbero considerare i benefici e gli svantaggi di dare il vaccino a pazienti estremamente fragili (come quelli la cui fragilità è classificata 8 o 9 sulla Clinical Frailty Scale o equivalente) o malati terminali.

Misure preventive come una buona idratazione, revisioni delle medicine e un trattamento ottimizzato delle condizioni di co-morbilità possono anche ridurre il rischio di conseguenze fatali da reazioni avverse ai vaccini, ha aggiunto il gruppo di esperti.

 

Al 18 maggio 2021, 155 decessi sono stati segnalati in Norvegia dopo la vaccinazione con il vaccino Pfizer-BioNTech, e la maggior parte dei decessi ha coinvolto anziani e molto fragili residenti in case di cura.2

Pfizer ha detto in una dichiarazione che tutte le decisioni sul lancio dei programmi di vaccinazione sono di competenza delle autorità sanitarie e che le autorità sanitarie norvegesi avevano precedentemente emesso raccomandazioni aggiornate sulla vaccinazione di malati terminali e pazienti estremamente fragili.

“Più di 300 milioni di dosi del vaccino Pfizer-BioNTech covid-19 sono state somministrate a livello globale”, ha detto Pfizer. “Ci si aspetta che gli eventi avversi gravi non collegati alla vaccinazione, ma vicini nel tempo, si verifichino in un tasso simile negli individui vaccinati come nella popolazione generale”.

Solo i vaccini BioNTech-Pfizer e Moderna fanno ora parte del programma di vaccinazione contro il covid-19 in Norvegia. L’11 marzo i funzionari hanno sospeso l’uso del vaccino AstraZeneca a causa delle preoccupazioni per i coaguli di sangue, e in aprile l’Istituto norvegese di salute pubblica ha raccomandato di non usare più quel vaccino.3

Geir Bukholm, direttore della Divisione di Controllo delle Infezioni e Salute Ambientale presso l’Istituto Norvegese di Sanità Pubblica, ha detto: “Poiché ci sono poche persone che muoiono di covid-19 in Norvegia, il rischio di morire dopo la vaccinazione con il vaccino AstraZeneca sarebbe più alto del rischio di morire per la malattia, in particolare per le persone più giovani.” Al 25 maggio, la Norvegia aveva registrato 782 morti per covid-19.4

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Michele Santoro: “In nessun tg si sente un medico scettico sul vaccino”. Bufera a DiMartedì

L’affondo del giornalista ieri sera durante il programma di La7. Floris lo gela, Sallusti gli dà del no-vax

Michele Santoro a DiMartedì
R.M. 26 maggio 2021 11:50
Fuoco e fiamme ieri sera a ‘DiMartedì’ tra Michele Santoro, Giovanni Floris e Alessandro Sallusti. A incendiare il conduttore e il direttore di Libero – ma non solo – la bomba lanciata dall’ex presentatore di ‘Annozero’ commentando l’informazione che si sta facendo in merito alla campagna vaccinale.”In quale telegiornale avete sentito parlare un medico scettico sul vaccino?” ha chiesto provocatoriamente Santoro, incalzando: “In quale telegiornale avete trovato un passante critico sulla politica di vaccinazione?”. E ancora: “I giornalisti dovrebbero controllare quello che fanno gli scienziati e i politici, ma se si trasformano da agenti di controllo in comunicatori, allora abbiamo un unico patto che tiene insieme giornalisti, scienziati e politici. Questo fatto è riduttivo per la democrazia. Si può accettare che in un tg non si veda mai uno che non è d’accordo con la campagna vaccinale? Ci sarà pure qualcuno che quando arriva il giornalista dica ‘non mi voglio vaccinare’?”.

Un attacco inaccettabile per il padrone di casa, che ha replicato: “Se parlo del globo terrestre non devo per forza avere un terrapiattista in studio”, mentre Sallusti dava del no-vax al collega ospite in studio. Risposte che non hanno placato il furore di Santoro: “Ho fatto il vaccino, sono assolutamente favorevole. Siccome dico che bisogna rappresentare l’opinione dei no-vax, divento no-vax. E’ un atteggiamento che non si può accettare – ha concluso – Quindi se sono no-vax non devo pagare il canone Rai, in quanto non ho diritto a veder rappresentata la mia opinione. Questo blocco ha reso inerte il dibattito democratico, quasi inutile. Se devo stare fermo a casa non è detto che la tv debba ammorbarmi con cose tutte uguali. Sono fermo? Allora fatemi vedere un dibattito”.

https://www.today.it/media/tv/santoro-vaccino-dimartedi.html

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È giunta l’ora! La Madre Addolorata si presenterà a tutta l’Umanità! Con le sue lacrime di sangue annunzierà l’evento disastroso che sta per colpire la Terra.

Pubblicato il

Carbonia 26-05-2021  –  ore 17.00

è giunta l’ora! La Madre Addolorata si presenterà a tutta l’Umanità!
Con le sue lacrime di sangue annunzierà l’evento disastroso che sta per colpire la Terra.

Maria SS.ma: Quale grazia figli miei, quale grazia per voi! Eccomi, il mio manto vi copre e vi protegge dal male.

Il doloroso evento che sta per verificarsi sulla Terra sarà distruttivo. Tutti coloro che non avranno accolto il grido di salvezza del Dio Amore, si troveranno in condizioni di pena.

Figli miei, questa è verità, quello che Io vi dico non è per il vostro male, ma è per il vostro bene! Voi non volete ascoltare il vostro Dio perché c’è un altro dio in mezzo a voi, c’è il dio di morte che voi venerate, adorate e seguite, un dio che presto vi porterà con sé dove mai più vedrete la luce, dove la sofferenza è eterna, lì brucerete nel fuoco del dolore, soffocherete nei vostri peccati.

Poveri figli miei, questo è quello che voi avete scelto, avete preferito servire un dio di morte anziché un Dio di vita eterna.

Il mio manto è aperto sui miei figli, essi sono ricoverati in Me, preziosi ai miei occhi sono i miei piccoli, i miei bambini, Io li custodirò con la forza di un leone! Io, la Madre Santissima, la Vergine Purissima, Madre di Dio e Madre vostra, vi porterò in Me, vi solleverò da ogni intemperia, niente vi colpirà di ciò che è male, perché, Dio ha preferito il vostro bene.

Gesù: L’Italia sta per entrare nella grande tribolazione! Non si ferma qui il dolore per gli italiani, inizia ora! L’Italia verrà smembrata completamente e venduta al nemico. Il falso profeta ha deciso di mettere la sua firma: un’alleanza definitiva con il nemico. L‘anticristo è già nella casa di Dio e siede sul trono di Pietro, povera Italia! Povera Chiesa mia caduta nelle miserie di Satana! I miei sacerdoti hanno abbracciato la Menzogna, hanno rifiutato il loro Dio Amore.

Gesù non c’è più nelle Chiese, figli miei, il tabernacolo è vuoto di Sé,
…non può Gesù unirsi a Satana!

Ma ancora per poco Satana gestirà nella Chiesa, perché dal Cielo discenderà nuovamente il Figlio dell’Uomo e tutto si irradierà di Luce e tutto tornerà nella Luce.

La Chiesa brillerà nell’amore di Dio, tornerà al suo Creatore e lo seguirà in assoluta fedeltà, …i suoi sacerdoti, i suoi nuovi sacerdoti, saranno eternamente fedeli al loro Dio Amore.

Figli miei, volgete ora lo sguardo verso l’alto e attendete il segno, tutto è imminente. Quando arriverà il segno sarà il momento della scelta definitiva, l’uomo che lo rifiuterà sarà soffocato dal male, …e pianto e stridore di denti sarà per lui.

è giunta l’ora! La Madre Addolorata si presenterà a tutta l’Umanità!
Con le sue lacrime di sangue annunzierà l’evento disastroso che sta per colpire la Terra.

Pregate figli miei, pregate e digiunate, allontanatevi dalle cose del mondo, non pensate più alle cose di questa Terra, convertitevi figli miei, convertitevi! Grande sarà il dolore che questa Umanità attraverserà se non tornerà al suo Dio Creatore.

Io, quale Madre di Gesù e Madre vostra vi chiedo umilmente di tornare al Padre vostro, di tornare ad abbracciare il santo Vangelo di Gesù e viverlo pienamente, vi chiedo di tornare alla Parola di Dio e seguire Dio, … non Satana!
La Terra e già avvolta nell’oscurità, ora si manifesteranno i segni e saranno tanti, si susseguiranno uno dietro l’altro, tutto ciò che vi è stato annunziato dai profeti di ieri e di oggi si manifesterà.

Figli miei, state per entrare nella fornace ardente e non vi preoccupate, continuate a sperare nel cambiamento, …vi illudete che tutto tornerà alla normalità, ma non sarà così, il mondo è nella morsa del veleno di Satana.

Gesù richiama a Sé i suoi figli, li benedice nel nome del Padre del Figlio dello Spirito Santo, e continua a segnare i suoi con il segno della croce sulla fronte e attende la conversione degli ultimi, dei ritardatari, per poterli salvare.
Avanti, non è più tempo di giocare, i giochi sono finiti, state per entrare nella grande buriana, non fatevi trovare impreparati per non impazzire, figli miei.
Allungo le mie mani e le mie braccia a voi, vi poso nel mio Petto e vi rallegro con l’amore di Dio, dal mio Petto scaturisce l’amore di mio Figlio Gesù, e verrà trasmesso a chiunque si farà abbracciare da Me.

L’ora è questa! Tutto è compiuto! L’assalto è predisposto!
Vi benedico nel nome del Padre, del Figlio e dello Spirito Santo.

Myriam: la dettatura si ferma, cala il silenzio ma il messaggio non è finito, avverto ancora la presenza della Vergine Santissima e non tardo a risentire il suo dire, …ecco la sua parola:

Maria SS.ma: Scenderà dal cielo una nube infuocata!
Una sfera di fuoco sta per colpire la Terra, figli miei, è la purificazione!
Ma, in molti non siete pronti, figli miei!
Questi sono gli ultimi tempi, gli ultimi atti che attraverserete su questa Terra in queste condizioni. Tutto verrà presto trasformato, il dolore sarà grande per chi non si sarà preparato.

Chiedete perdono in fretta!!! …chiedete perdono in fretta!!!
Inginocchiatevi davanti al Crocifisso, chiedete perdono, perdono, perdono per i vostri peccati, per le vostre miserie infinite, per l’atrocità che vige in questo mondo a causa dell’uomo che si è venduto al nemico, a Satana.

Vi amo, voglio tutti salvarvi, voglio prendervi tutti sotto il mio manto; …venite, Io vi proteggerò. Entrate nell’Arca, Io sono la nuova Arca, figli miei, …la nuova Arca! Tutti vi ricovererò in Me se deciderete di scegliere mio Figlio Gesù a Satana!

Le porte Io apro! …Vi apro il mio manto e vi apro il mio Petto! …Non dite di no, fatevi salvare. Asciugate le lacrime di questa Mamma che tanto vi ama, per asciugare le vostre lacrime che, amare saranno se non prenderete la decisione di ritornare al vostro Dio Amore, lacrime amare che dovrete versare.

Sia lodato Gesù Cristo.

How to Overcome the Pressure to Get a COVID Vaccine…

Posted on by

vac These Last Days News – May 27, 2021
URGENT: Forward a link to this web page to your clergy, family, friends and relatives.

How to Overcome the Pressure to Get a COVID Vaccine…

“Doctors of the earth, what have you done to yourselves in your practices? You do not seek to preserve life, but to destroy.” – Our Lady, March 18, 1973

MASTERS OF DECEIT
“Be known it to you that it has been written of and spoken of as 666, the masters of deceit. Satan has entered into the highest places of your government, of each nation of the world, to sow confusion, discontentment, atheism, communism, satanism, and all manner of evil.” – Jesus, October 2, 1980

THE OCTOPUS OF EVIL
“The octopus of evil having many arms now is reaching out. The evil and the agents of satan in human form have entered into the highest places in world governments and in My Son’s Church.” – Our Lady, March 18, 1977

The above Messages from Our Lady were given to Veronica Lueken at Bayside, New York. Read more

LifeSiteNews.com reported on May 27, 2021:

by Christian Elliot

Recently a friend of mine who adamantly did NOT want to get a COVID vaccine, did so anyway because of pressure from her peers and her employer.

For any of you who feel pressured to get a COVID jab and feel like it’s becoming impossible to say no, I’ve got your back.

Before I’m done you will:

  1. Understand why vaccine mandates and coercion are illegal.
  2. Have a practical way to converse about your decision to opt-out.
  3. Feel confident in your decision, and (I hope) feel emboldened to speak up.

Side note: In case you missed my recent article on 18 reasons I won’t be getting a COVID vaccine, you might check it out if you want additional talking points beyond what’s below.

Dealing with employer & school mandates

If I were an employee or student who was required or feeling coerced into getting a COVID vaccine, here’s how I would strategically handle it…

Ask for exemptions

Politely, and with a curious tone, ask what exemptions are in place for people who need to decline the shot?

If you get any pushback for asking that question, you can kindly say that is between you and your doctor.

Hopefully, you’ll be presented with how to file/qualify for the allowed exemptions and that will be the end of the story.

If asking for exemptions does not prove to be fruitful, here’s what I would do next.

Point out liability

Pharma can’t be sued for injuries or deaths caused by their vaccines, but companies, schools, and individuals that mandate them, can be.

If your institution is trying to mandate or coerce you into taking something against your will, not only is that 100% ILLEGAL (more on that below), by forcing you to take a product, they make themselves liable if you get injured or die.

No organization wants to hear that, but given the way our government is promoting the COVID “vaccines,” it is understandable that most institutions don’t even know that mandates are illegal, nor do they understand the liability mandates expose them to.

To give you legal and ethical ground to stand on, let’s start here…

Vaccine mandates for experimental COVID shots are against the law in the US.

  • You can see the actual law in this letter sent to all Universities currently trying to mandate the COVID shot.
  • Mandates (and shockingly many of the government sponsored vaccine ads) go against FTC law regarding deceptive advertising.
  • Mandates create all sorts of problems with HIPPA (medical-privacy) law – in case you care to see the list of ongoing lawsuits that have arisen when people’s HIPPA rights are violated, you can click here.

Furthermore, coercion tactics are in violation of U.S. and International Law.

The opening frame of the Nuremberg Code—written after WWII to make sure no one is ever again forced to participate in medical interventions without their consent—states this:

The voluntary consent of the human subject is absolutely essential.

This means that the person involved should have legal capacity to give consent; should be so situated as to be able to exercise free power of choice, without the intervention of any element of force, fraud, deceit, duress, over-reaching, or other ulterior form of constraint or coercion; and should have sufficient knowledge and comprehension of the elements of the subject matter involved, as to enable him to make an understanding and enlightened decision.

Did you catch that part about: “without…any coercion”?

In actuality, these mandates, and the entire COVID narrative violate all 10 items of the Nuremberg code.

If you want to see what a group of 1,000 lawyers and 10,000 doctors are doing to highlight these issues and begin new crimes-against-humanity trials, you can click here.

The short version: The good guys are punching back.

The bottom line: Mandates and coercion are ILLEGAL.

Period.

If your school or employer goes to court and is found guilty of breaking these very-clear laws, they will lose.

Let that sink in…and now let’s talk about how to practically…

Stand up for your rights

Here are two tactics you can consider to help you point this out the problems with mandates?

  • Good Cop: You could mention this liability issue as a way to show you are looking out for the best interest of your school or employer—i.e. you could be the hero that keeps them out of court, helps craft a respectful policy, and saves them countless dollars in legal battles.
  • Bad Cop: You could overtly mention litigation and that you would be happy to file a lawsuit if anything happens to you or your peers. This may or may not be good for office politics (or your education/career path), but if you’re in a position of leverage this may be the fastest way to sway in your organization’s policy.

Before I get to how I might handle peer-pressure or medical-pressure, permit me a…

Critical thinking interlude

Given the above, I’d argue there are some critically important questions to ask:

  • Why are our governments literally spending billions of taxpayer dollars to overtly coerce all of us to take these experimental products?
  • Are they ignorant of the law (seems implausible) or are they actively, knowingly engaged in something illegal?
  • Why is the White House not quick to point out to businesses that mandates and coercion are against the law?
  • Why is the FTC not cracking down on illegal and deceptive advertising? – Why is it left to non-profits to take our institutions to task?
  • Why are donuts, cash, or reestablishing pre-covid privileges (like going to a ballpark in NY without a vaccine and sitting where you used to) not at least being frowned up as manipulation, or discrimination, if not overt coercion?

It’s not as if the federal government, and even some states, are being subtle about trying to get everyone to take these “vaccines.”

They are shoving it on us, and using every well-honed tactic of fear, guilt, shame, and attempts to withhold freedoms that they can come up with.

If you don’t think this is happening, as kindly as I can say it, you’re not paying attention.

This propaganda is happening from the White House podium and our taxpayer dollars are funding the largest, coercion-based, “vaccine” promoting, media blitz in history.

In case you missed it, here’s a recent quote from Joe Biden?

“The rule is now simple: get vaccinated or wear a mask until you do. The choice is yours.”

I’m not a lawyer, but that sure sounds like coercion Mr. President!

…and you’re not even being subtle about it.

Shame on you.

  • You are breaking the Nuremberg code.
  • You are contributing to the division in this nation.
  • You are being dismissive of the risks these products carry.
  • You’re tying our freedoms to compliance with what you think it best.
  • You are stepping into the middle of a private decision between us and our doctors.
  • You are unraveling trust in science, medicine, and our government.

When did you become so emboldened as to blatantly violate the law?

Are you actually ignorant of the law, or do you know better and do this anyway?

It makes me wonder if you’re a President…or a puppet.

Side note: I’m saving my thoughts on the wizards behind the curtain for another post about how you can talk me out of my “conspiracy theory.”

For now, here’s the point…

If our federal government can unashamedly break the law, encourage social media titans to censor all dissent, suppress or withhold all non-vaccine treatments, and erode our freedoms, who then is actually looking out for us?

This isn’t a gray area. Mandates and coercion are black-and-white illegal.

Even if you want to get the “vaccine” (fine, that’s your choice), does it not bother you to see what our federal government is doing?

If clear examples of government overreach don’t bother you, I’m guessing (I could be wrong) this is because they have worn you down by taking away so many of your freedoms that you’re willing to make this intellectual compromise just so life can “go back to normal.”

Am I right?

Dear friends, if you think giving up your freedoms has ever resulted in getting them back, you don’t know history.

The only way we take back our freedoms is to fight for them.

As sincerely as I can ask: At what point will you look at the bigger picture and say “OK, this has crossed an unethical line”?

What more would have to happen before you say that?

Even if you like the so-called “vaccine,” what is your line in the sand for unethical government overreach, and when will you stand up and do something about it?

OK…interlude over…back to how to effectively push back against pressure to get a “vaccine.”

Find legal help

Given that our leaders aren’t likely to be held accountable or change their tune in the next few months, let’s turn our attention back to the immediately practical.

If I’m in your shoes…and the above didn’t cause your school or employer to (quickly) remove the mandate, or at the very least hastily find an exemption, I’d make sure to kindly submit (in writing) my objection to being forced to take it, and let it be known that if I am forced to do so, or if my employment is threatened, I would have to consider legal action.

If you need help finding an attorney to pushback against your school or employer, here are some resources for you:

  • Informed Consent Action Network (ICAN) – In order to obtain potential legal assistance, email ICAN at freedom@icandecide.org and provide a copy of the written notice from your school or employer stating that the COVID-19 vaccine is required. You can also see this letter ICAN is sending to all universities mandating vaccines.
  • America’s Frontline Doctors, Legal Eagle Dream Team (love that name) – has put together fantastic letters you can share with your employer or school to put the fear of God in them by showing them how untenable a vaccine mandate is and the scary volume of liability they would have if they tried to.
  • Children’s Health Defense – has put together this simple one-page letter explaining the law to any employer or school that attempts to mandate the COVID vaccine.
  • Health Freedom Defense Fund – They aid families and individuals whose health rights have been infringed and they support legal challenges to unjust laws that undermine our health and freedoms. You can contact them and see a list of resources here. Their legal team can also send Cease-and-Desist letters on your behalf if needed.
  • State-by-State List of Vaccine Attorneys. If you need help for a more specific situation, you can check out this list of attorneys standing by to help.
  • For those living in Canada, you might be able to find legal help here.

I’m sure there are other places you can find legal help and letters to submit to your organization (if you know of more please let me know and I will add them), but the above should get you started.

Now let’s turn our attention to…

Dealing with medical or peer pressure

If you are under pressure from anyone in the medical profession to get a covid jab, here are some ways I might handle it.

#1: FIRE YOUR DOCTOR

Doctors work for you, not the other way around.

If a healthcare professional is pressuring you to take the shot, politely (or confrontationally if that’s your style) fire that doctor or nurse and find a new one.

There are plenty of doctors who are happy to protect (and champion) your right to decline the shot.

If your relationship with your doctor (or a friend/peer) is one you want to keep and you’d rather take more of a bridge-building/educational approach, you can pivot to this strategy:

#2: ASK THE UNANSWERABLE QUESTION

As I detailed in my last post, there are so many show-stopper, inconvenient, exposing questions to ask about these “vaccines,” but let me give you the simplest one that is probably all you need.

Ask for a Lab Test to Screen You for Susceptibility to Vaccine Injury

In other words, ask your doctor (or friend) what type of lab tests are available to make sure that your body (or someone with your health condition) is not at risk for having any of the known, heart-wrenching, life-altering injuries detailed in this video from the Informed Consent Action Network.

If you didn’t watch the above video montage yet, please find the time.

Those stories are just a fraction of the ones that the powers-that-be would prefer to sweep under the rug in their unabashed vaccine propaganda.

The point is…

None of the people in the montage above were tested for counter-indications that would have been a red flag for a possible vaccine injury.

Why?

Because testing for susceptibility doesn’t happen.

The best “screening” I could find is this laughable, propaganda document from the CDC that basically says unless you have a severe allergic reaction to polyethylene glycol (or your first COVID jab), you should be good to go.

The bottom line: There is no test your doctor can give you to screen you for possible complications.

Not one.

Let that sink in.

If you don’t believe me, ask for a test.

The pushback you may get…

If you ask the unanswerable question, you’ll likely get the standard, canned reply… “Not to worry, the vaccines are safe and effective, and the risks are one-in-a-million.” (BTW, injuries are way more than one in a million).

In case you get that predictable reply, here’s all you have to do—repeat your question.

Say, “I get it doc, but how do we know MY body is not at risk for becoming injured”?

An honest doctor (or nurse/pharmacist, etc.) will tell you there’s no way to know.

No modestly-informed person, certainly no medical professional, disputes that all medical products carry risks.

Furthermore, is there any medical professional or public-health leader who denies that these shots have injured or killed people?

From blood clots, to menstrual issues, to Bell’s palsy, to anaphylaxis’, body-wide hives, life-altering tremors, and death, these vaccines carry very real risks.

#3: LET MEDICAL PROFESSIONALS DO SOME REASONING FOR YOU

In case you’re reasoning with someone in the medical profession, let me drive home the unanswerable question with a video interview of three nurses who were devastatingly injured when they got a COVID “vaccine.”

All three nurses: 

  • Suffer from debilitating tremors.
  • Are unable to work, thus they lost their income.
  • Are unable to drive.
  • Get no worker’s compensation.
  • Have no recourse to sue the manufactures.
  • Have mountains of debt from all their medical bills.
  • Have been abandoned by their own medical profession.
  • Can’t file an insurance claim because their situation isn’t covered.
  • Have to rely of family members to meet their most basic and personal needs.
  • Blame themselves for not “doing their homework.”

Two of the nurses didn’t even know the Vaccine Adverse Events Reporting System (VAERS) existed before they took the shot.

One of them even said if she had been presented with the “anecdotal” information about all the risks, she would have still gotten the shot.

Do you suppose she thinks differently now?

Do you think she wouldn’t take back her choice in a heartbeat?

These nurses were all “pro-vaccine,” obviously.

They all “trusted the science” and now have been left with injuries that may be lifelong.

How do you know this wouldn’t happen to you?

Is this the time for blind faith, or is it the time for tougher questions?

#4: OTHER INCONVENIENT QUESTIONS YOU CAN ASK

In case you need any other questions to reason with your friends or medical professionals, here are some other talking points to choose from:

  • Ask when the clinical trials for these “vaccines” ended? Hint: They haven’t. They aren’t schedule to be over until near the end of 2022 or sometime in 2023.
  • Ask to see the results of the long-term safety studies. Hint: There aren’t any. How could there be?
  • Ask to see the “package insert.” What’s that? It’s an informative piece of paper that should come with every shot. If you can find one, you can ask questions like:
    • What does it mean “There is no FDA-approved Covid-19 vaccine?” (p.1), or
    • Why doesn’t it list “stopping transmission or infection of the ‘SARS-CoV-2 virus’” as one of the benefits (p.3)? Hint: that’s because SARS-CoV-2 is the virus–Covid-19 is the symptoms you manifest. Accidental omission? Hardly.
    • Ask when we’ve ever used an unapproved vaccine (or any vaccine) in the middle of an outbreak. Hint: We never have. Why not? What problems could that cause?
  • Especially if you’ve already had COVID: Ask when have we ever vaccinated people for a disease they have already overcome? Hint: We never have. Why would we do that now? What risks does that present?
  • Ask what other medical product has ever been prescribed for every medical condition, every gender, and (almost…they’re working on it) every age? Hint: Not one. So, what makes these products something that is “safe and effective” for everyone, without even adjusting the dosage regardless of how old you are or how much you weigh?
  • Ask if they’ve seen any of these 18 reasons not to get the shot.

You might even curiously point out that:

In 1976, our government stopped a rushed vaccine program (for H1N1) because:

  • 450 (of 45,000,000 who were vaccinated) reported developing Guillain-Barre syndrome (.00001%)
  • 30+ people died (less than .000001%)

For comparison sake…

(NOTE: The numbers below rely on a reporting system that is notorious for capturing only a small fraction (1%) of known adverse events, Nonetheless, let’s compare the best data we can find from US and EU government agencies.)

That’s probably a few more clotting disorders than you heard about in the news…

…and yet the COVID-vaccine agenda marches on.

What would it take for us to finally be alarmed at the carnage these vaccines are causing?

If you formally ask the CDC such questions, be prepared for a long silence.

With all that said, let’s step back from logical arguments and get personal.

Dealing with the emotional loss of family, friends, and freedom

Now, you may be thinking, “OK, I get it—the vaccines carry real risks and the mandates are illegal.”

BUT, if I cause a ruckus or say no to the shot, I’m also saying no to seeing my family and friends, I’m likely losing my ability to travel, and I may have a pick an uncomfortable fight with my work/school, etc.

If that’s you, perhaps this quote from Benjamin Franklin can be of value:

“He who gives up a little freedom to gain a little security, deserves neither and will lose both.”

Yes, your life, and your choices in the near term may get annoyingly, unpalatably disrupted.

Your family may refuse to talk to you.

You may have to find new friends.

You may travel less…for a while.

But, what is better, handing over your freedom and gambling that you won’t get injured, or adjusting your strategy and expectations, finding new friends, and fighting for your freedom.

Which choice will you look back on and say was the right choice?

Here are two things I can tell you:

  • Given that demand for the COVID jab is plummeting, the propaganda engine is kicking into high gear. The pressure is not going to let up anytime soon. You’re either going to cave, or find your backbone.
  • You are NOT alone. The truth-loving, science-protecting, freedom fighters are not cowering in their basements. I’ve been encouraged to meet so many of the them (doctors, nurses, scientists, and activists) since writing this post that went viral.

In addition to knowing you’re not alone, be encouraged by looking at the revolutions of history that have overthrown tyranny.

What you find is that there’s a good chance we don’t even need a majority. If (from the estimates I’ve seen) 13-20% of us will get loud, organized, and stand up for truth, we win this!

Look, I know it takes courage to stand in the fire.

I know these conversations are uncomfortable, but do you have a better option?

As I said above, once they take our freedom, they won’t give it back.

If we cave in now, there will be more mandates, and more erosion of our freedoms.

Here’s the beautiful thing about speaking truth and asking genuinely-thoughtful questions — there’s no need to be rude, or bombastic; truth just needs to be spoken.

People will recognize truth when they hear it.

Where can you let your voice be heard and stand up for what’s right?

In conclusion

I hope you found this both practically helpful and inspiring.

Don’t be bullied into compromising your integrity and succumbing to an illegal mandate.

The time to stand up for truth is upon us. It may get uncomfortable, but so be it.

For all of you who’ve felt too fearful to speak out, we need you.

Find the courage to stand in the fire and join the fight.

Truth is going win!

https://www.tldm.org/news50/how-to-overcome-the-pressure-to-get-a-covid-vaccine.htm

Valutazione degll’effetto immunogenico di nanoparticelle superparamagnetiche (YFe2O3@Ag) come piattaforma per lo sviluppo e il controllo di adiuvanti nei vaccini

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Responsabili di progetto
Stefano Lupi, Asmaa Mohamed Salman
Accordo
EGITTO – NRC – National Research Centre of Egypt
Bando
CNR/NRC biennio 2018-2019 2018-2019
Dipartimento
Scienze fisiche e tecnologie della materia
Area tematica
Scienze fisiche e tecnologie della materia
Stato del progetto
Nuovo

Proposta di ricerca

Background: The unique physicochemical properties of Nanoparticles (NPs) enable their potential applications in several biomedical fields. In particular, super paramagnetic NPs have been applied in drug discovery, gene delivery, imaging, photodynamic therapy and tissue engineering. Standard toxicity studies of NPs in biological systems are essential component of NPs safety profiling. To this end, the Egyptian group synthesized super paramagnetic YFe2O3@Ag NPs and assessed their toxicity both in vitro and preclinically in vivo, using traditional toxicological assessment and biochemical parameters [the study was funded by Science and Technology Development Fund (STDF), project title: Optimization and characterization of the biosynthesis of metal nanoparticles for medical application, ID number 12649]. The results showed that 100 mg/kg is a safe NP dose as assessed by pathological and biochemical parameters. However, preliminary studies with Fourier transform infrared imaging of tissue sections of mice treated with the aforementioned NPs provided evidence for detectable changes in the biochemical profile of the tissues (including alterations of protein folding, nucleic acids and carbohydrates content) mainly in organs of the immune system (namely spleen, bone marrow and blood) at sub-cytotoxic concentrations. This work has been presented as a poster at the 9th International Conference on Advanced Vibrational Spectroscopy; ICAVS 2017, in Victoria (Canada) by Dr. Eid. Thus, the in vivo and in vitro biochemical and pathological studies together with ex-vivo FTIR Imaging suggest that YFe2O3@Ag nanocomposite could possibly behave as a safe immunomodulator. Relying on purely biochemical signatures rather than on labeling with exogenous dyes and stains, infrared chemical imaging has the potential to greatly enhance the reliability of micro-morphological and/or biochemical assays.This demand is particularly severe for NPs, since their nano-size induces unpredictable interaction with the most common dyes, leading to contradictory and often unreliable results. Conversely, label-free FTIR imaging provides in a single spectrum and comprehensive overview of the biochemical sample profile, giving information on concentration and structure of biological relevant macromolecules and also on their spatial distribution at the micrometer scale.The use of dimensional Focal Plane Array detectors allow for the fast imaging of large samples, such as tissues, while Synchrotron Radiation (SR) FTIR point mapping enhance the spectral quality for minute and low-absorbing samples, such as individual cells.
The present project aims to gain insight into the effect of YFe2O3@Ag NPs at sub-cytotoxic concentrations on the biochemical profile of immune organs (inguinal, axillary, brachial and cervical lymph nodes, and thymus), combining ex-vivo FTIR Imaging with the investigation of cytokine secretions and standard in vivo and in vitro assays. In addition, we aim to validate the hypothesis that YFe2O3@Ag NPs can be used as a carrier for in vitro screening of vaccine adjuvants, exploiting single cell Synchrotron Radiation FTIR microscopy, and correlating information from conventional cell-based assays on lymphocytes and peritoneal macrophages.
The project goal will be achieved through a collaborative research that involves complementary scientific team from Egyptian and Italian CNR Institutions. The pharmacology and physics team from National Research Centre, Egypt will be responsible for preparation of the NPs and their biological evaluation in vitro and in vivo. The staff of the infrared beamline at Elettra Sincrotrone Trieste, from both CNR and Elettra branches, will contribute to this project providing instrumentation for performing both tissue ex vivo and cellular in vitro vibrational analyses. Expertise in bio-spectroscopy and data analysis will be shared with the Egyptian team, aiming to create a synergy that will last beyond the project limits.
Methodology: Specifically the objectives of the present proposal will be addressed in three steps. Step 1: In vivo and ex vivo testing of the effect of administering 100 mg/kg YFe2O3@Ag NPs on the immune system of mice: i. Animal treatment and isolation of immune organs. All animal procedure of the proposed work is enclosed under ethical approval sought for ongoing project (Identifying novel natural immunomodulatory therapeutics using in vitro and in vivo approaches) funded by National Research Centre (NRC) (project number: 11010310, ethical permit number: 16090); ii.Evaluation of inflammatory markers and immunoglobulins. Step2: Evaluation of spleen derived lymphocytes and peritoneal macrophage functions in response to YFe2O3@Ag NPs treatment in vitro based on viability, reactive oxygen species (ROS) production and cytokine levels. Step 3: Explore the use of YFe2O3@Ag NPs as a carrier for vaccine adjuvants (bacterial lipopolysaccharide and Polyinosinic-polycytidylic acid) by in vitro screening of NP effects on peritoneal macrophages using SR-FTIR microspectroscopy. i.Loading of lipopolysaccharide (LPS) and Polyinosinic-polycytidylic acid (Poly I:C) onto YFe2O3@Ag; ii. Validating the use of YFe2O3@Ag NPs as a carrier of vaccine adjuvants (LPS and Poly I:C) by SR-FTIR microspectroscopy.
Expected outcomes: 1. Demonstration of the immunological response induced by YFe2O3@Ag NPs in in vivo and in vitro models. 2. Assessment of the potentials of YFe2O3@Ag NPs as a carrier of vaccine adjuvants. 3. Strengthening the international collaboration between Egypt and Italy in the framework of NRC-CNR agreement that goes beyond the specific projects.

Obiettivi della ricerca

The project goal will be achieved by the fulfillment of the following objectives:
1. Assessment of the impact on mice immune organs – including inguinal, axillary, brachial and cervical lymph nodes, thymus and spleen – of administering 100 mg/kg ³Fe2O3@Ag NPs by complementing in vivo and ex vivo studies, using biochemical markers and FTIR imaging.
2. Evaluation of the immune responses to ³Fe2O3@Ag NPs treatment in spleen derived lymphocytes and peritoneal macrophage functions in vitro
3. Investigation of the potential use of ³Fe2O3@Ag NPs as a carrier of vaccine adjuvants (namely, bacterial lipopolysaccharide and Polyinosinic-polycytidylic acid) and their potential of immunogenicity, through in vitro screening of the effects of adjuvant-NP conjugates on peritoneal macrophages using SR-FTIR microscopy complemented by cytokine signature analysis.

Ultimo aggiornamento: 27/05/2021